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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified E B IH

Catalog #	B1750-16
Applications	Suitable for Western Blot, ELISA and Immunohistochemistry. Other applications have not been tested.
Recommended Dilution	ELISA: 1:500 to 1:2500
	Optimal dilutions to be determined by the researcher.
Storage and Stability	Store at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	F(ab’)2
Host	Goat
Concentration	~0.5mg/ml (UV280)
Form	Supplied as a liquid in 0.05M Tris chloride, 0.15M sodium chloride, 0.001M Magnesium Chloride, 0.001M zinc chloride, pH 8.0, 10mg/ml BSA, 0.01% sodium azide and 50% glycerol. Labeled with alkaline phosphatase.
Purity	Purified by immunoaf nity chromatography.
Immunogen	Biotin conjugated to Keyhole Limpet Hemocyanin (KLH).
Specificity	Recognizes Biotin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-AP, anti-goat serum and biotinylated IgG. No reaction was observed against anti-pepsin or anti-goat IgG F(c).
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.