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You are here:Home » Antibodies » Abs to Assay Detection » Anti -Biotin (AP)

Anti -Biotin (AP)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH
Catalog #B1750-16
ApplicationsSuitable for Western Blot, ELISA and Immunohistochemistry. Other applications have not been tested.
Recommended DilutionELISA: 1:500 to 1:2500
Optimal dilutions to be determined by the researcher.
Storage and StabilityStore at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
CAS NumberN/A
Clone TypePolyclonal
IsotypeF(abí)2
HostGoat
Concentration~0.5mg/ml (UV280)
FormSupplied as a liquid in 0.05M Tris chloride, 0.15M sodium chloride, 0.001M Magnesium Chloride, 0.001M zinc chloride, pH 8.0, 10mg/ml BSA, 0.01% sodium azide and 50% glycerol. Labeled with alkaline phosphatase.
PurityPurified by immunoaf nity chromatography.
ImmunogenBiotin conjugated to Keyhole Limpet Hemocyanin (KLH).
SpecificityRecognizes Biotin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-AP, anti-goat serum and biotinylated IgG. No reaction was observed against anti-pepsin or anti-goat IgG F(c).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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