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You are here:Home » Antibodies » Antibodies-Vitamins » Anti -Biotin (Cy2)

Anti -Biotin (Cy2)


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Clone Host Grade Applications
Polyclonal Goat Affinity Purified B IF
Catalog #B1750-17
ApplicationsSuitable for use in Immunoflourescence and FLISA (Fluorescent). Other applications not tested.
Recommended DilutionImmunoflourescence: 1:100-1:1000
Optimal dilutions to be determined by the researcher.
Absorption Wavelength489nm
Emission Wavelength:
Fluorochrome/Protein Ratio:
2.1 moles Cy2 per mole of goat IgG F(abí)2
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
FormSupplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Cy2 (Cyanine 2-OSu - Molecular Weight
PurityPurified by immunoaffinity chromatography.
ImmunogenBiotin (KLH)
SpecificityRecognizes Biotin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, biotin conjugated goat IgG and biotin conjugated BSA. No reaction was observed against anti-goat IgG F(c) or anti-Pepsin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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