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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified B IF

Catalog #	B1750-17
Applications	Suitable for use in Immunoflourescence and FLISA. Other applications not tested.
Recommended Dilution	Immunoflourescence: 1:100-1:1000
	Optimal dilutions to be determined by the researcher.
Absorption Wavelength	489nm
	Emission Wavelength:
	505nm
	Fluorochrome/Protein Ratio:
	2.1 moles Cy2 per mole of goat IgG F(ab’)2
Storage and Stability	Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	F(ab’)2
Host	Goat
Concentration	~1mg/ml
Form	Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Cy2 (Cyanine 2-OSu - Molecular Weight
Purity	Purified by immunoaffinity chromatography.
Immunogen	Biotin (KLH)
Specificity	Recognizes Biotin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, biotin conjugated goat IgG and biotin conjugated BSA. No reaction was observed against anti-goat IgG F(c) or anti-Pepsin.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.