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You are here:Home » Antibodies » Abs to Vitamins » Anti -Biotin (Cy3.5)

Anti -Biotin (Cy3.5)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E
Catalog #B1750-17A
ApplicationsSuitable for use in ELISA. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Absorption Wavelength 581nm
Emission Wavelength: 596nm
Fluorochrome/Protein Ratio: 2.2 moles Cy3.5 per mole of Goat IgG F(abí)2
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeF(abí)2
HostGoat
Concentration~1mg/ml
FormSupplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA, (IgG and Protease free), 0.01% Sodium Azide.
PurityPurified by immunoaffinity chromatography.
ImmunogenBiotin conjugated to Keyhole Limpet Hemocyanin (KLH)
SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, biotin conjugated Goat IgG and biotin conjugated BSA. No reaction was observed against anti-Goat IgG F(c) or anti-Pepsin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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