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You are here:Home » Antibodies » Abs to Vitamins » Anti -Biotin (Cy3)

Anti -Biotin (Cy3)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E IF
Catalog #B1750-17B
ApplicationsSuitable for use in ELISA (Fluorescent) and Immunofluorescence. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Absorption Wavelength 550nm
Emission Wavelength 570nm
Fluorochrome/Protein Ratio 5.4 moles Cy3 per mole of Goat IgG F(abí)2
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypePolyclonal
IsotypeF(abí)2
HostGoat
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, (IgG and Protease free), 0.01% sodium azide. Labeled with Cy3.
PurityPurified by immunoaffinity chromatography.
ImmunogenBiotin conjugated to Keyhole Limpet Hemocyanin (KLH).
SpecificityRecognizes biotin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, biotin conjugated goat IgG and biotin conjugated BSA. No reaction was observed against anti-goat IgG F(c) or anti-Pepsin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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