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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified IF

Catalog #	B1750-17B
Applications	Suitable for use in FLISA and Immunofluorescence. Other applications not tested.
Recommended Dilution	Optimal dilutions to be determined by the researcher.
Absorption Wavelength	550nm
Emission Wavelength	570nm
Fluorochrome/Protein Ratio	5.4 moles Cy3 per mole of Goat IgG F(ab’)2
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	F(ab’)2
Host	Goat
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, (IgG and Protease free), 0.01% sodium azide. Labeled with Cy3.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Biotin conjugated to Keyhole Limpet Hemocyanin (KLH).
Specificity	Recognizes biotin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, biotin conjugated goat IgG and biotin conjugated BSA. No reaction was observed against anti-goat IgG F(c) or anti-Pepsin.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.