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You are here:Home » Antibodies » Abs to Vitamins » Anti -Biotin (Cy5)

Anti -Biotin (Cy5)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IF
Catalog #B1750-17E
ApplicationsSuitable for use in ELISA and Immunofluorescence. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Excitation Wavelength 650nm
Emission Wavelength 667nm
Fluorochrome/Protein Ratio 5.3 moles Cy5 per mole of goat IgG F(abí)2. Molecular weight of label: 975D.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
Clone TypePolyclonal
IsotypeF(abí)2
HostGoat
Concentration~1mg/ml
FormSupplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Cy5. Reconstitute with 1ml sterile ddH2O.
PurityPurified by immunoaffinity chromatography.
ImmunogenBiotin conjugated to Keyhole Limpet Hemocyanin (KLH).
SpecificityRecognizes a single precipitin arc against anti-goat serum, biotin conjugated goat IgG and biotin conjugated BSA. No reaction was observed against anti-goat IgG F(c) or anti-pepsin.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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