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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified E B IF

Catalog #	B1750-17E
Applications	Suitable for use in ELISA and Immunofluorescence. Other applications not tested.
Recommended Dilution	Optimal dilutions to be determined by the researcher.
Excitation Wavelength	650nm
Emission Wavelength	667nm
Fluorochrome/Protein Ratio	5.3 moles Cy5 per mole of goat IgG F(ab’)2. Molecular weight of label: 975D.
Storage and Stability	Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	F(ab’)2
Host	Goat
Concentration	~1mg/ml
Form	Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Cy5. Reconstitute with 1ml sterile ddH2O.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Biotin conjugated to Keyhole Limpet Hemocyanin (KLH).
Specificity	Recognizes a single precipitin arc against anti-goat serum, biotin conjugated goat IgG and biotin conjugated BSA. No reaction was observed against anti-goat IgG F(c) or anti-pepsin.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.