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You are here:Home » Antibodies » Antibodies-Epitope Tags » Anti -Biotin (D-Biotin, Vitamin H, Coenzyme R, Bioepiderm) (FITC)

Anti -Biotin (D-Biotin, Vitamin H, Coenzyme R, Bioepiderm) (FITC)


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Clone Host Grade Applications
Polyclonal Goat Affinity Purified IH IC FC IF
Biotin is used as a growth factor in mammalian cell culture as well as having numerous immunological purification roles in avidin/streptavidin-biotin binding mechanisms.
Catalog #B1750-20A
ApplicationsSuitable for use in Immunoelectrophoresis, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, F.I.T., FLISA and Flow Cytometry/FACS. Other applications not tested.
Recommended DilutionImmunohistochemistry: 1:20-1:2000
Immunocytochemistry: 1:50-1:5000
F.I.T.: Mean fluorescence intensity of a 1:1000 dilution at Ex-488nm (+/-9)/Em-518nm (+/-9) = 6643 MFI’s
FLISA: Mean fluorescent value of 8ug/ml of FITC conjugate when tested against biotinylated Chicken IgG/Y coated at 4ug/ml = 1558 MFI’s
Optimal dilutions to be determined by the researcher.
ConjugateFluorescein-5-isothiocyanate (FITC) Amax = 494nm; Emax = 518nm; F/P Ratio = 8.1:1
Storage and StabilityStore product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. 
Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypePolyclonal
FormSupplied as a in 10mM sodium phosphate, 0.15M sodium chloride, pH 7.2, 1% BSA (protease/IgG free), 0.05% sodium azide. Labeled to fluorescein isothiocyanate (FITC)
PurityAffinity purified using solid phase biotin. ~95% based on SDS-PAGE.
ImmunogenPurified Biotin
SpecificityRecognizes free Biotin and Biotin coupled to BSA, KLH or IgG/Y. Two predominant bands are seen at 50 and 24kD in SDS-PAGE. Does not react with free BSA or free KLH.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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