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You are here:Home » Antibodies » Abs to Assay Detection » Anti -Biotin (D-Biotin, Vitamin H, Coenzyme R, Bioepiderm) (HRP)

Anti -Biotin (D-Biotin, Vitamin H, Coenzyme R, Bioepiderm) (HRP)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B IH Gel
Catalog #B1750-30
ApplicationsSuitable for use in Immunoblot (Western or Dot Blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays. Other applications not tested.
Recommended DilutionELISA: 1:5000 to 1:25,000
Western Blot: 1:500- 1:3000.
Immunohistochemistry: 1:300- 1:1500.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
Concentration~1mg/ml (UV280)
FormSupplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, 0.01% gentamicin sulfate, 40% glycerol. Labeled with HRP.
PurityPurified by immunoaf nity chromatography.
ImmunogenBiotin conjugated to Keyhole Limpet Hemocyanin (KLH)
SpecificityRecognizes Biotin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, biotinylated bovine serum albumin and Biotinylated IgG.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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