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Specifications

Clone Host Grade Applications Polyclonal Goat Affinity Purified IF

Catalog #	B1750-35A
Applications	Suitable for use in Immunofluorescence Microscopy. Other applications not tested.
Recommended Dilution	Immunofluorescence: 1:500-1:2000
	Optimal dilution determined by the researcher.
Absorption Wavelength	596 nm
	Emission Wavelength: 620 nm
Fluorochrome/Protein Ratio	3.1 moles Texas Red per mole of goat IgG
Storage and Stability	Lyophilized powder may be stored at 4°C. Reconstitute with sterile ddH2O. Aliquot and store at -20°C. Reconstituted product is stable for 12 months as an undiluted liquid. Dilute only prior to immediate use. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Polyclonal
Host	Goat
Concentration	~1.1mg/ml (after reconstitution)
Form	Supplied as a powder lyophilized from a solution containing PBS, pH 7.2, 10mg/ml BSA (Ig and protease-free), and 0.01% sodium azide. Labeled with Texas Red. Reconstitute with 1ml ddH2O.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)
Specificity	Recognizes Biotin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, biotinylated bovine serum albumin and biotinylated IgG.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.