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Anti -Biotin (D-Biotin, Vitamin H, Coenzyme R, Bioepiderm)
Anti -Biotin (D-Biotin, Vitamin H, Coenzyme R, Bioepiderm)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Monoclonal |
Mouse |
Affinity Purified |
E B |
|
| Catalog # | B1750-38 | | Applications | Suitable for use in ELISA, Western Blot. Other applications not tested. | | Recommended Dilution | ELISA: 1:20,000 | | Optimal dilutions to be determined by the researcher. | | Hybridoma | 3X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice. | | Culture Medium | RPMI 1640 with 10% fetal calf serum | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | n/a | | Clone Type | Monoclonal | | Isotype | IgG2a,k | | Clone No | 6F40 | | Host | Mouse | | Concentration | ~0.5mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide, before the addition of glycerol to 40%. | | Purity | Purified by Protein A/G affinity chromatography from culture supernatant. | | Immunogen | Biotinylated denatured ovalbumin. | | Specificity | Recognizes and reacts strongly with biotin. Strong reaction is seen in ELISA with biotin/OA directly coated onto the microtiter well, also when tested in sandwich ELISA in combination with streptavidin. In Western blot, after SDS-PAGE, reacts with biotinylated proteins in both reduced and non-reduced forms. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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