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You are here:Home » Antibodies » Abs to Assay Detection » Anti -Biotin (AP) (Vitamin H, Coenzyme R, Bioepiderm)

Anti -Biotin (AP) (Vitamin H, Coenzyme R, Bioepiderm)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified E B
Catalog #B1750-42
Application Optimized for the detection of biotinylated protein molecular weight standards.
ApplicationsSuitable for use in ELISA, Western Blotting. Other applications not tested.
Recommended DilutionWestern Blotting: 1:1000-1:3000. The alkaline phosphatase (AP) conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent or other substrates for detection on Western blots. One of the advantages for AP conjugation is that the reaction rate remains linear for a long period of time.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
FormSupplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, BSA, 50% glycerol. Conjugated to calf intestinal alkaline phosphatase.
PurityPurified by immunoaffinity chromatography.
ImmunogenBiotin
SpecificityBiotin
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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