Login

Forgot your password?
New User?
Remember me
banner banner

You are here:Home » Biochemicals » Biochem-Vitamins » Biotin (Vitamin H, Coenzyme R, Bioepiderm) (Glucose Oxidase)

Biotin (Vitamin H, Coenzyme R, Bioepiderm) (Glucose Oxidase)

Pricing

  For pricing information, USA customers sign in.
  Outside USA? Please contact your distributor for pricing.

Specifications

Biotin is used as a growth factor in mammalian cell culture as well as having numerous immunological purification roles in avidin/streptavidin-biotin binding mechanisms. Biotin, also known as vitamin H or B7, is a water-soluble B-complex vitamin which is composed of an ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a cofactor in the metabolism of fatty acids and leucine, and it plays a role in gluconeogenesis. Biotin is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids. It plays a role in the citric acid cycle, which is the process by which biochemical energy is generated during aerobic respiration. Biotin not only assists in various metabolic reactions, but also helps to transfer carbon dioxide. Biotin is also helpful in maintaining a steady blood sugar level. Biotin is often recommended for strengthening hair and nails. Consequently, it is found in many cosmetic and health products for the hair and skin. Deficiency is extremely rare, as intestinal bacteria generally produce an excess of the body's daily requirement.
Catalog #B1750-45
ApplicationsSuitable for use in ELISA, Western Blot, Dot Blot, Immunohistochemistry as well as other Glucose Oxidase-biotin-avidin based enzymatic assays.. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, dH2O.r. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PurityPurified by ion exchange chromatography. IEP Assay shows a single precipitin arc against anti-Glucose Oxidase and anti-Biotin.
Concentration~5mg/ml (UV280)
FormSupplied as a lyophilized powder in PBS, pH 7.2. No preservative added. No stabilizing proteins added. Reconstitute in 1ml of 40-50% glycerol, dH2O.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


External Links