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You are here:Home » Antibodies » Abs to Binding Proteins » Anti -BiP

Anti -BiP

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Rabbit Supernatant B IH FC
Secretory and transmembrane proteins are synthesized on polysomes and translocated into the endoplasmic reticulum (ER). Inside the ER, these proteins are often modified by disulfide bond formation, amino-linked glycosylation and folding. To help proteins fold properly, the ER contains a pool of molecular chaperones including BiP. BiP was identified as an immunoglobulin heavy chain binding protein in pre-B cells. It was also found to be induced at the protein level by glucose starvation. When protein folding is disturbed inside ER, BiP synthesis is increased. Subsequently, BiP binds to misfolded proteins to prevent them from forming aggregates and assists them to refold properly.
Catalog #B1770
ApplicationsSuitable for use in Western Blot, Immunohistochemistry and Flow Cytometry. Other applications not tested.
Recommended DilutionsWestern Blot: 1:1000
Immunohistochemistry (Paraffin):1:200 (Antigen retrieval: Citrate/TBST-5%NGS)
Immunohistochemistry (Frozen): 1:200 (10% neutral buffered formalin)
Flow Cytometry: 1:100
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG
Clone No7H9
HostRabbit
SourceHuman
ConcentrationNot Determined
FormSupplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
PuritySupernatant
ImmunogenSynthetic peptide corresponding to residues surrounding Gly584 of human BiP.
SpecificityRecognizes endogenous levels of total human BiP protein at ~78kD. Species Crossreactivity: mouse
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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