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Specifications

Clone Host Grade Applications Monoclonal Mouse Ascites E B IH IC

BLK ( B lymphoid tyrosine kinase), with 505-amino acid protein (about 56KDa), belongs to the Src non-receptor tyrosine kinases familDifferent subcellular localizations of Src-family kinases may be important for the regulation of specific cellular processes such as mitogenesis, cytoskeletal organization, and membrane trafficking.Blk is expressed exclusively by B lymphocytes and it is thought to function in a signal transductory pathway specific to this lineage. B lymphoid expression of an active Blk mutant caused proliferation of B progenitor cells and enhanced responsiveness of these cells to interleukin 7. Thus, sustained activation of Blk induces responses normally associated with the pre-BCR.
Catalog #	B1900-02
Applications	Suitable for use in ELISA, Western Blotting, Immunohistochemistry. Other applications not tested.
Recommended Dilution	ELISA: 1: 10,000
	Western Blot: 1: 500- 1: 2,000
	Immunohistochemistry-Paraffin: 1: 500- 1: 2,000
	Immunohistochemistry-Frozen: 1: 500- 1: 2,000
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a
Clone No	9j109
Host	Mouse
Source	Human
Form	Ascites
Purity	Ascites
Immunogen	Ni-NTA purified truncated recombinant BLK expressed in E. coli strain BL21 (DE3)
Specificity	Recognizes human BLK (B lymphoid tyrosine kinase).
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.