| Human BLAME (B-lymphocyte activator macrophage expressed), also known as SLAM family member 8, is a type I transmembrane protein that belongs to the CD2 subset of immunoglobulin superfamily cell receptors. CD2 family proteins function as adhesion molecules and modulators of immune responses. Mature human BLAME consists of a 211aa ECD that contains two Ig V-like domains, a 21aa transmembrane segment, and a 31aa cytoplasmic tail that lacks recognizable signaling motifs (3). Within the ECD, human BLAME shares |
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| 19-26% aa sequence identity with human 2B4, CD2, CD2F-10, CD48, CD58, CD84, CD229, CRACC, NTB-A, and SLAM. It shares 79% aa sequence identity with the ECD of mouse BLAME. BLAME is expressed on dendritic cells and IFN- stimulated monocytes. Overexpression of BLAME in bone marrow cells leads to an increase in the peritoneal B1b population of B lymphocytes. |
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| Catalog # | B2106 |
| Molecular Mass | The recombinant human BLAME is a monomeric protein. Based on N-terminal amino acid sequencing, the recombinant protein starts at Ala 23 and it has a calculated molecular mass of 24.7kD. As a result of glycosylation, the recombinant monomer migrates as an approximately 35-40kD protein in SDS-PAGE under reducing conditions. |
| Endotoxin | 1.0 EU per 1ug of the protein as determined by the LAL method. |
| Biological Activity | Measured by its ability to bind biotinylated rhBLAME in a functional ELISA type binding assay. |
| Storage and Stability | Aliquot to avoid repeated freezing and thawing and freeze at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months. |
| Molecular Weight | 24.7kD |
| Source | The extracellular domain of human BLAME (aa23-233; Accession # NP_064510) fused to six histidines at the C-terminus expressed in NSO cells. |
| Purity | >90% as determined by SDS-PAGE and visualized by silver stain. Endotoxin: 1.0 EU per 1ug of the protein as determined by the LAL method. |
| Form | Supplied as a lyophilized powder from 20mM citrate, 0.3M sodium chloride, pH 6.0. Reconstitute with sterile PBS to a concentration of 0.1mg/ml. |
| Specificity | Recognizes human BLAME. Within the ECD, human BLAME shares 19-26% aa sequence identity with human 2B4, CD2, CD2F-10, CD48, CD58, CD84, CD229, CRACC, NTB-A, and SLAM. Species sequence homology: mouse 79%. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
