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You are here:Home » Antibodies » Abs to Membrane Proteins » Anti -BLAME (B-lymphocyte Activator Macrophage Expressed)

Anti -BLAME (B-lymphocyte Activator Macrophage Expressed)

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Purified E FC
B Lymphocyte Activator Macrophage-Expressed (BLAME), also known as SLAMF8, is an Ig superfamily protein that is part of the CD2 family of receptors. It is expressed on macrophages and dendritic cells. BLAME modulates signaling through the B-cell receptor and may also function during B-lineage commitment.
Catalog #B2106-02
ApplicationsSuitable for use in Flow Cytometry and ELISA. Other applications not tested.
Recommended DilutionsFlow Cytometry: 25ug/ml. Use 10ul to label 1-2.5x10e5 cells in a total reaction volume not exceeding 200ul.
Direct ELISA: 0.5-1.0ug/ml. Detection limit is ~5ng/well.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1
Clone No8H128
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenNS0-derived recombinant human BLAME extracellular domain (aa23-233; Accession # Q9P0V8)
SpecificityRecognizes human BLAME. Does not react with recombinant human SLAMF7 or recombinant human SLAMF6.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesSLAMF8


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