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Specifications

X-a-Gal is a chromogenic substrate for a-galactosidase (also known as melibiase or alpha-D-galactoside galactohydrolase), an enzyme which enables yeast to use the disaccharide melibiose as a carbon source during growth or fermentation. In the yeast Saccharomyces cerevisiae this enzyme is encoded by the MEL1 gene which is regulated by several GAL genes. Secretion of this enzyme in response to GAL4 activation leads to hydrolysis of X-a-Gal in the medium causing yeast colonies to develop a blue color. A big advantage of this reporter system is that it abolishes the need for b-galactosidase filter-lift assays and the result can be seen immediately on the selection plates.
Catalog #	B2175-05
Storage and Stability	Store powder at -20°C under nitrogen. Powder is stable for 12 months at -20°C.
	Aliqout and store stock solutions of X-a-Gal at -20°C. Aliquots are stable for at least 6 months at -20°C. Protect from light and humidity.
Molecular Formula	C14H16BrNO6
Molecular Weight	374.2
Purity	98% (HPLC)
Appearance	White to off white powder.
Solubility	1% in DMF is clear and practically colorless
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate names	5-Bromo-3-Indolyl-a-D-galactoside (galactopyranoside)