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You are here:Home » Molecular Biology » MB-Enzymes, Restriction » Bme1390I (ScrFI)

Bme1390I (ScrFI)

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Specifications

5'-C C^N G G-3'
3'-G G N^C C-5'
Catalog #B2180
Concentration 10u/ul
Source Bacillus megaterium RFL1390
Buffer 50mM Tris-HCl (pH 7.5), 10mM MgCl2, 100mM NaCl and 0.1mg/ml BSA. Incubate at 37°C
Diluent Buffer 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol, or Storage Buffer.
Storage Buffer 10mM Tris-HCl (pH 7.5 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% glycerol.
Overdigestion AssayNo detectable change in the specific fragmentation pattern is observed after 320-fold overdigestion (20u/ug DNA x 16 hours) with Bme1390I.
Ligation/Recutting AssayAfter 50-fold overdigestion (3u/ug DNA x 17 hours) with Bme1390I, more than 80% of the DNA fragments can be ligated in a reaction mixture containing 20–40u of T4 DNA ligase/1ug of fragments and 10% PEG at a 5'-termini concentration of 1.8uM. More than 90% of these can be recut.
Labeled Oligonucleotide (LO) AssayNo detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10units of restriction endonuclease for 4 hours.
Methylation EffectsBme1390I does not cut Cm5CNGG. Blocked by overlapping Dcm or CG methylation.
Stability during Prolonged IncubationA minimum of 0.2units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37°C.
Thermal InactivationEnzyme is inactivated by incubation at 80°C for 20min.
Compatible EndsCC^(C/G)GG–BcnI, SatI CC^(A/T)GG–MvaI, SatI
Number of Recognition Sites in DNA
Lambda184
PhiX1743
M13mp18/1911
pBR32216
pUC18/1912
pUC5712
pTZ19R/U10
pBluescriptIIKS(-/+)11
pBluescriptIISK(-/+)11
pACYC17714
pACYC18422
Unit DefinitionOne unit is defined as the amount of enzyme required to digest 1ug of lambda DNA dcm-in 1 hour at 37°C in 50ul of assay buffer.


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