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You are here:Home » Antibodies » Abs to Transcription Factors, Zinc (Ring) Finger » Anti -Bmi-1, IN (RNF51, MGC12685, BUP, Polycomb group RING finger protein 4)

Anti -Bmi-1, IN (RNF51, MGC12685, BUP, Polycomb group RING finger protein 4)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Bmi1, a transcriptional repressor, also known as RNF51, MGC12685, or BUP, is a transcriptional repressor that encodes a set of polycomb complex protein bmi-1 family members (1). The molecule is involved in segment specification, cell growth and/or maintenance, transcription regulation, and chromatin modification. A critical target of Bmi-1 is the INK4a locus, which
encodes the p16 and p19ARF (p14ARF in humans) tumor suppressor proteins (2). Overexpression of Bmi-1 extends the replicative life spans of mouse and human fibroblasts, possibly by repressing p16 and p19/p14ARF. A study using mouse embryo fibroblasts (MEFs) harboring specific deletions in the INK4a locus indicates that p19ARF, but not p16, is required for replicative senescence of mouse cells (3). Bmi 1 is located in nuclear and expressed on brain (cerebellar cortex) and unregulated in many malignant diseases like ovary cancer, and lymphoma.
Catalog #B2185-01
ApplicationsSuitable for use in ELISA and Western Blot. Other applications have not been tested.
Recommended DilutionELISA: 0.1-0.5ug/ml
Western Blot: 1-3ug/ml
Optimal dilutions to be determined by the researcher.
Positive ControlHuman K562 cells
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
Clone TypeMonoclonal
IsotypeIgG1,k
Clone No6A10
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide derived from the mid region of the human Bim-1 protein.
SpecificityRecognizes human Bim-1 at ~45kD.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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