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You are here:Home » Antibodies » Abs to Transcription Factors, Zinc (Ring) Finger » Anti -Bmi-1 (Polycomb complex protein BMI-1, Polycomb group RING finger protein 4, RING finger protein 51)

Anti -Bmi-1 (Polycomb complex protein BMI-1, Polycomb group RING finger protein 4, RING
finger protein 51)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified B IP
he Bmi-1 protein is a member of the polycomb group (PcG) proteins. Bmi-1 has been reported as an oncogene by regulating the cell cycle inhibitors p16 and p19. Bmi-1 knockout in mice results in defects in hematopoiesis, skeletal patterning, neurological functions, and development of the cerebellum. Bmi-1 is necessary for efficient self-renewing cell divisions of adult hematopoietic stem cells as well as adult peripheral and central nervous system neural stem cells. Bmi-1 may regulate the self-renewal of other types of somatic stem cells, and it is also thought to inhibit aging in neurons through the suppression of p53. Several lines of evidence implicate Bmi-1 in tumorigenesis. Differential expression of Bmi-1 in a mouse model of metastatic prostate cancer vs. wild-type suggests Bmi-1 expression levels may predict response to therapy and survival in multiple types of cancer.
Catalog #B2185-03C
ApplicationsSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.
Recommended DilutionWestern Blot: 1:5000
Optimal dilutions to be determined by the researcher.
Positive ControlU20S Cell Lysate
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG1k
Clone No9H201
HostMouse
SourceMouse
Concentration~1mg/ml
FormSupplied as a liquid in 0.1M Tris-Glycine, pH7.4, 150mM sodium chloride, 0.05% sodium azide.
PurityPurified by Protein G affinity chromatography.
ImmunogenRecombinant fusion protein of full length murine BMI-1 tagged with MBP.
SpecificityRecognizes murine BMI-1 (~35kD). Species Crossreactivity: human.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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