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You are here:Home » Antibodies » Abs to Binding Proteins » Anti -BD-1 (Beta-defensin 1, mBD-1, Defb1)

Anti -BD-1 (Beta-defensin 1, mBD-1, Defb1)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Antimicrobial peptides are a common mechanism of host defense utilized by a variety of species, from insects to humans. Defensins are a large family of broad-spectrum antimicrobial peptides, identified originally in leukocytes of rabbits and humans. Defensins, cationic/polar peptides (30-35aa; 3-4kD), are distinguished by a conserved tri-disulfides and a largely beta-Sheet structure. Defensins, expressed at the cell surface, have been hypothesized to function as a biochemical barrier against microbial infection by inhibiting colonization of the epithelium by wide range of pathogenic microorganisms.
Catalog #B2469-02D
The genes encoding human alpha and beta-defensins are clustered in a contiguous segment of chromosome 8p23. Defensins are classified into two families designated and based on distinctive, although similar, tri-disulfide linkages in the peptides. beta-defensins are slightly larger and differ in the position and arrangement of 3 disulfides. In humans, six alpha-defensin (cryptidins), HD 1-6 (HD1-4 are also known as HNP1-4 for Human Neutrophil Peptides), and two beta-defensins, HBD-1 and HBD-2, have been identified to date. Rat RBD-1 and RBD-2) and mouse (MBD1-4) homologues of the human beta-defensin have also been identified. alpha-defensins are encoded by genes designated DEFA1-6, whereas human beta-defensins are encoded by the DEFB1 and DEFb2 genes.
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: 0.5-1ug/ml
Western Blot: 1-10ug/ml using ECL technique
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostRabbit
SourceMouse
ConcentrationAs reported
FormSupplied as a lyophilized powder from PBS, 0.1% BSA.
PurityPurified by immunoaffinity chromatography
ImmunogenKLH-conjugated synthetic 16 amino acid peptide sequence within the N-terminal region of aa33-69 of mature mouse beta-defensin 1 (UniProt Accession #P56386). This sequence is 87% conserved in rat BD-1 and no significant homology exists with human beta-defensin 1 or other defensins.
SpecificityRecognizes mouse MBD-1
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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