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Bone Morphogenetic Protein 2 (BMP2), Human, Recombinant
Bone Morphogenetic Protein 2 (BMP2), Human, Recombinant
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| Belongs to the Bone-growth regulatory factors that are members of the transforming growth factor-beta (TGF-beta) superfamily of proteins. They are synthesized as large precursor molecules which are cleaved by proteolytic enzymes. The active form can consist of a dimer of two identical proteins or a heterodimer of two related bone morphogenetic proteins. | | | Catalog # | B2553-06A | | Recombinant human Bone Morphogenetic Protein-2 produced in E. coli is a homodimeric, non-glycosylated polypeptide chain containing 115 amino acids. | | Amino Acid Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Met-Gln-Ala-Lys-His. | | Biological Activity | The ED50 as determined by the cytolysis of MC3T3-E1 cells is <50ng/ml corresponding to a specific activity of 2x10e4 IU/mg. | | Protein Content | Protein quantitation was carried out by two independent methods: | | 1. UV spectroscopy at 280nm using the absorbency value of 1.4 as the extinction coefficient for a 0.1% (1mg/ml) solution. | | 2. Analysis by RP-HPLC, using a standard solution of BMP2 as a Reference Standard. | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 100mM acetic acid. Add 0.1% HSA or BSA for long term storage. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | 26018D | | Source | E. coli | | Purity | 95% by RP-HPLC and SDS-PAGE Silver Stain. Chromatographically purified. | | Form | Supplied as a powder lyophilized from 10mM sodium citrate, pH 3.5. Reconstitute with sterile 100mM acetic acid to 0.1mg/ml. Add 0.1% HSA or BSA for long term storage. This solution can then be diluted into other aqueous solutions. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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