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You are here:Home » Molecular Biology » Growth Factors-BMP » Bone Morphogenetic Protein 2 (BMP2), Human, Recombinant

Bone Morphogenetic Protein 2 (BMP2), Human, Recombinant

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Specifications

Belongs to the Bone-growth regulatory factors that are members of the transforming growth factor-beta (TGF-beta) superfamily of proteins. They are synthesized as large precursor molecules which are cleaved by proteolytic enzymes. The active form can consist of a dimer of two identical proteins or a heterodimer of two related bone morphogenetic proteins.
Catalog #B2553-06A
Recombinant human Bone Morphogenetic Protein-2 produced in E. coli is a homodimeric, non-glycosylated polypeptide chain containing 115 amino acids.
Amino Acid SequenceThe sequence of the first five N-terminal amino acids was determined and was found to be Met-Gln-Ala-Lys-His.
Biological ActivityThe ED50 as determined by the cytolysis of MC3T3-E1 cells is <50ng/ml corresponding to a specific activity of 2x10e4 IU/mg.
Protein ContentProtein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280nm using the absorbency value of 1.4 as the extinction coefficient for a 0.1% (1mg/ml) solution.
2. Analysis by RP-HPLC, using a standard solution of BMP2 as a Reference Standard.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 100mM acetic acid. Add 0.1% HSA or BSA for long term storage. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight26018D
SourceE. coli
Purity95% by RP-HPLC and SDS-PAGE Silver Stain. Chromatographically purified.
FormSupplied as a powder lyophilized from 10mM sodium citrate, pH 3.5. Reconstitute with sterile 100mM acetic acid to 0.1mg/ml. Add 0.1% HSA or BSA for long term storage. This solution can then be diluted into other aqueous solutions.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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