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You are here:Home » Antibodies » Abs to Collagen » Anti -Bone Morphogenetic Protein 4 (BMP4, DVR4)

Anti -Bone Morphogenetic Protein 4 (BMP4, DVR4)


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Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Catalog #B2553-22
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: To detect hBMP-4 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 4µg/ml of this antibody is required. This monoclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.1ng/well of recombinant hBMP-4.
Western Blot: To detect hBMP-4 by Western Blot analysis this antibody can be used at a concentration of 0.25ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBMP-4 is 3.91ng/lane, under non-reducing conditions.
Optimal dilutions to be determined by the researcher.
ReconstitutionReconstitute to 1mg/ml with sterile dH2O or sterile 40-50% glycerol for long term storage.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Country of OriginUSA
Clone TypeMonoclonal
Clone No7F2
FormSupplied as a lyophilized powder from PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenPurified recombinant, E. coli derived, human BMP-4
SpecificityRecognizes human Bone Morphogenic Protein 4.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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