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You are here:Home » Molecular Biology » Growth Factors-BMP » Bone Morphogenetic Protein 7 (BMP7, OP-1, Osteogenic Protein-1) Human, Recombinant

Bone Morphogenetic Protein 7 (BMP7, OP-1, Osteogenic Protein-1) Human, Recombinant

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Specifications

BMP-7 (osteogenic protein 1) belongs to the Bone-growth regulatory factors that are members of the transforming growth factor-beta (TGF-beta) superfamily of proteins. They are synthesized as large precursor molecules which are cleaved by proteolytic enzymes. The active form can consist of a dimer of two identical proteins or a heterodimer of two related bone morphogenetic proteins.
Catalog #B2553-32
Recombinant Human BMP-7 is a monomeric, non-glycosylated, Polypeptide chain containing 139 amino acids and having a molecular mass of 15.68kD.
SequenceThe sequence of the first five N-terminal amino acids was determined and was found to be Ser-Thr-Gly-Ser-Lys.
ApplicationsSuitable for use in Western Blot, ELISA, preparing antibodies for BMP-7 monomer and as a molecule standard in detecting secreted BMP-7 in reduced SDS-PAGE. Other applications not tested.
Storage and StabilityLyophilized powder may be stored at -20°C. Reconstitute with sterile 20mM acetic acid. Aliquot and store at -20°C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer with 0.1% HSA or BSA.
Molecular Weight15.68kD
SourceRecombinant, Human (E. coli).
Purity95% by RP-HPLC or SDS-PAGE. Chromatographically purified.Chromatographically purified.
Endotoxin0.1ng/ug (IEU/ug)
Concentration~1mg/ml
FormSupplied as a lyophilized powder in 10mM sodium citrate, pH 3.5. Reconstitute with sterile 20mM acetic acid to 0.1mg/ml. This solution can then be diluted into other aqueous solutions with 0.1% HSA or BSA.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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