| Bone Morphgenetic Protein 9 (BMP-9), also known as GDF-2, is a member of the BMP subgroup of the TGF-beta superfamily proteins. BMP-9 regulates the development and function of a variety of embryonal and adult tissues. It also promotes hepatic lipid metabolism and inhibits glucose production. Unlike with other BMP family proteins, the propeptide remains associated with the mature peptide after proteolytic cleavage and does not interfere with BMP-9 biological activity. Mature human and mouse BMP-9 share 96% aa sequence identity. |
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| Applications: |
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| Suitable for use in Western Blot, ELISA and Neutralization. Other applications not tested. |
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| Catalog # | B2553-36 |
| Recommended Dilution | Western Blot: 1ug/ml using recombinant human BMP-9 under non-reducing conditions only |
| ELISA: 2-8ug/ml (Capture); 0.1-0.4ug/ml (Detection) |
| Neutralization of human BMP-9 bioactivity: Measured by its ability to neutralize BMP-9-induced alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. The Neutralization Dose (ND50) is typically 0.01-0.04ug/ml in the presence of 2ng/ml Recombinant Human BMP-9. |
| Optimal dilution determined by the researcher. |
| Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG2b |
| Clone No | 7H136 |
| Host | Mouse |
| Source | Human |
| Concentration | ~0.5mg/ml (after reconstitution) |
| Form | Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS. |
| Purity | Purified by Protein G affinity chromatography. |
| Endotoxin | < 0.1EU/ug (LAL). |
| Immunogen | Recombinant corresponding to aa320-429 from human BMP-9, expressed in E.coli (Accession# Q9UK05). Species Sequence Homology: mouse (96%) |
| Specificity | Recognizes human Bone Morphogenetic Protein 9. Does not cross react with recombinant human BMP-1, -1.1, -2, -3, -3b, -4, -5, -6, -7, -8 or -10. In direct ELISAs, ~80% cross-reactivity with recombinant mouse BMP-9 and 10% cross-reactivity with recombinant mouse BMP-10 is observed. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
