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You are here:Home » Molecular Biology » Growth Factors-BMP » Bone Morphogenetic Protein 10, Recombinant, Human (BMP10) (BSA Free)

Bone Morphogenetic Protein 10, Recombinant, Human (BMP10) (BSA Free)

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Specifications

The mature recombinant human BMP-10, generated by the proteolytic removal of the signal peptide and the propeptide, is a disulfide-linked homodimeric protein. Based on N-terminal sequencing, the mature recombinant human BMP-10 starts at Asn 317. The monomer has a calculated molecular mass of 12.2kD and migrates as an approximately 12.2kD protein in SDS-PAGE under reducing conditions.
Catalog #B2553-37AX
SourceA DNA sequence encoding human BMP-10 (Met 1-Arg 424; Accession # O95393) was expressed in a Chinese hamster ovary (CHO) cell line.
Endotoxin< 1.0 EU per 1 g of the protein as determined by the LAL method.
ActivityMeasured by its ability to induce alkaline phosphatase synthesis by MC3T3E1 osteoblastic cells (Mazerbourg, S. et al., 2005, J. Biol. Chem. 280:32122). The ED50 for this effect is typically 15-60ng/mL.
ReconstitutionIt is recommended that sterile PBS be added to the vial to prepare a working stock solution of no less than 100ug/ml. The carrier-free protein should be used immediately upon reconstitution to avoid losses in activity due to non-specific binding to the inside surface of the vial. For long term storage as a dilute solution, a carrier protein (e.g. 0.1% HSA or BSA) should be added to the vial.
StorageLyophilized samples are stable for up to twelve months at -20°C. Upon reconstitution, this protein can be stored under sterile conditions at 2-8°C for one month or at -20°C in a manual defrost freezer for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
SourceChinese hamster ovary (CHO) cells
Purity97%, as determined by SDS-PAGE and visualized by silver stain.
ConcentrationAs reported
FormSupplied as a lyophilized powder in 30% acetonitrile, 0.1% TFA.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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