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Bone Morphogenic Protein 14, Human, Western Blot Control (BMP14, CDMP1, Cartilage-Derived Morphogenetic Protein-1, GDF-5)
Bone Morphogenic Protein 14, Human, Western Blot Control (BMP14, CDMP1, Cartilage-Derived
Morphogenetic Protein-1, GDF-5)
Pricing
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| Recombinant human GDF-5 produced in E. coli is a homodimer, non-glycosylated, polypeptide chain containing two 117aa and having a total molecular mass of 26.8kD. | | | To enable bacterial expression of rhGDF-5 we removed the N-terminal sequence Ala-Pro-Leu-Thr and added the Lysine. | | | Catalog # | B2553-51A | | Endotoxin | <0.1EU/ug (LAL) | | Biological Activity | Human GDF-5 is fully biologically active when compared to standard. The ED50, determined by the ability to induce alkaline phosphatase activity in ATDC5 cells is 20-30ng/ml. | | Amino Acid Sequence | APSATRQGKR PSKNLKARCS RKALHVNFKD MGWDDWIIAP LEYEAFHCEG LCEFPLRSHL EPTNHAVIQT LMNSMDPEST PPTCCVPTRL SPISILFIDS ANNVVYKQYE DMVVESCGCR | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. For long-term storage add 0.1% BSA or HSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | CAS Number | N/A | | Molecular Weight | 26.8kD | | Source | Recombinant, protein corrrsponding to human Bone Morphogenic Protein 14, expressed in E. coli | | Purity | 98% as determined by HPLC; Reducing and non-reducing SDS-PAGE. | | Form | Supplied as a lyophilized powder. Reconstitute with sterile ddH2O to a concentration of 0.1mg/ml, which can be further diluted into other aqueous solutions. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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