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You are here:Home » Growth Factors, Cytokines » Growth Factors-BMP » Bone Morphogenic Protein 14, Recombinant, Human, Western Blot Control (BMP14, CDMP1, Cartilage-Derived Morphogenetic Protein-1, GDF-5)

Bone Morphogenic Protein 14, Recombinant, Human, Western Blot Control (BMP14, CDMP1,
Cartilage-Derived Morphogenetic Protein-1, GDF-5)

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Specifications

Recombinant human GDF-5 produced in E. coli is a homodimer, non-glycosylated, polypeptide chain containing two 120aa and having a total molecular mass of 27.4kD. To enable bacterial expression of rhGDF-5 we removed the N-terminal sequence Ala-Pro-Leu-Thr and added the Lysine.
Catalog #B2553-51A
Endotoxin <0.1EU/ug (LAL)
Biological ActivityHuman GDF-5 is fully biologically active when compared to standard. The ED50, determined by the ability to induce alkaline phosphatase activity in ATDC5 cells is 20-30ng/ml.
Amino Acid SequenceAPSATRQGKR PSKNLKARCS RKALHVNFKD MGWDDWIIAP LEYEAFHCEG LCEFPLRSHL EPTNHAVIQT LMNSMDPEST PPTCCVPTRL SPISILFIDS ANNVVYKQYE DMVVESCGCR
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. For long-term storage add 0.1% BSA or HSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight27.4kD
SourceRecombinant, protein corrrsponding to human Bone Morphogenic Protein 14, expressed in E. coli
Purity98% as determined by HPLC; Reducing and non-reducing SDS-PAGE.
FormSupplied as a lyophilized powder. Reconstitute with sterile ddH2O to a concentration of 0.1mg/ml, which can be further diluted into other aqueous solutions.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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