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You are here:Home » Antibodies » Abs to Proteins » Anti -Bora (Protein Aurora Borealis, HsBora, C13orf34)

Anti -Bora (Protein Aurora Borealis, HsBora, C13orf34)

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Specifications

Clone Host Grade Applications
Polyclonal Goat Affinity Purified IC
Bora is a 61kD member of the Bora family of proteins. It is ubiquitously expressed, and plays a key role in cell cycle progression. Plk1 is a phosphorylase that is important to the cell during the G2/M transition and mitosis. Its activity is initially regulated by Aurora-A, which phosphorylates and activates Plk1 on aa210. Bora, Aurora-A and Plk1 all appear to form a complex during G2. Bora predisposes Plk1 to the actions of Aurora-A. Once activated by Aurora-A, Plk1 drives the mitotic mechanism, which includes a third-party phosphorylation of Bora. This initiates Bora dissociation from Aurora-A with subsequent ubiquitination and degradation. Human Bora is 559aa in length. It contains a Ser-rich region aa188-278 and at least eight utilized Ser phosphorylation sites. Phosphorylation may increase the SDS-PAGE MW of Bora to 75-85kD. There is one potential alternative start site that lies 60aa upstream of the standard start site, and a second splice variant the shows a 17aa substitution for aa1-87. Over aa2-180, human Bora shares 84% aa identity with mouse Bora.
Catalog #B2557-01
ApplicationsSuitable for use in Immunocytochemistry. Other applications not tested.
Recommended DilutionImmunocytochemistry: 5-15ug/ml
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
IsotypeIgG
HostGoat
SourceHuman
Concentration~0.1mg/ml after reconstitution
FormSupplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.2mg/ml.
PurityPurified by immunoaffinity chromatography.
ImmunogenRecombinant corresponding to aa2-180 from human Bora expressed in E.coli.
SpecificityRecognizes human Bora.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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