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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E B IF

Bordetella pertussis is an aerobic, gram negative coccobacilli. It causes whooping cough (pertussis), an acute respiratory infection marked by severe, spasmodic coughing episodes during the paroxysmal phase. Leukocytosis with lymphocytosis is also common during this phase of the illness. Dangerous complications are bronchopneumonia and acute encephalopathy. It produces a number of virulence factors, including pertussis toxin, adenylate cyclase toxin, filamentous hemagglutinin, and hemolysin. Agglutinogens and other outer membrane proteins are important antigens. Pertussis toxin (islet activating protein) is the major protein toxin produced by virulent strains of Bordetella pertussis. As revealed by polyacrylamide gel electrophoresis, the purified protein consists of five dissimilar subunits: S1 (MW 28kD), S2 (MW 23kD), S3 (MW 22kD), S4 (MW 11.7kD) and S5 (MW 9.3kD), in a molar ratio of 1:1:1:2:1. The A protomer, S1 is responsible for the enzymatic activity of the toxin. Together, S2, S3, S4 and S5 comprise the B oligomer, responsible for binding the toxin to the cell surface.
Catalog #	B2558-50
Applications	Suitable for use in Immunofluorescence, ELISA and Western Blot. Other applications not tested.
Recommended Dilution	Immunofluorescence: 1:10-1:50
	ELISA: 1:20-1:200
	Western Blot: 1:10-1:50
	Optimal dilutions to be determined by the researcher.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG3
Clone No	5E222
Host	Mouse
Concentration	~0.1mg/ml
Form	Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. No stabilizing proteins have been added.
Purity	Purified by Protein A chromatography ( >90%).
Immunogen	Bordetella pertussis
Specificity	Recognizes LOS-A of Bordetella pertussis and B. bronchiseptica. Antibody is bactericidal for Bordetella pertussis only.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.