| Islet-activating protein Pertussis toxin consists of an A protomer subunit (S1) which possesses both NAD+ glycohydrolase and ADP-ribosyltransferase activities, and B oligomer subunits (S2, S3, S4, and S5) which are responsible for attachment of the native toxin to eukaryotic cell surfaces. Pertussis toxin uncouples G proteins from receptors by ADP ribosylating a cysteine residue near the carboxyl terminus of the a subunit. |
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| Catalog # | B2558-55 |
| This preparation migrates as five distinct bands when analyzed by SDS-Urea PAGE. The five bands correspond to one A protomer subunit, designated S1 (Mr=26.2kD), and four B oligomer subunits, designated S2-S5 (Mr’s= 21.9, 21.9, 12.1, and 10.9kD, respectively). |
| Reconstitution | Reconstitute with 500ul sterile ddH2O. Because Pertussis toxin is relatively insoluble, the resulting suspension should be made uniform by gentle mixing prior to withdrawing aliquots. Do not sterile filter. |
| This preparation is not activated. For use with intact cells or extracts, activation is not necessary. However, when used with purified G proteins, this preparation should be activated according to the procedure of Kaslow et al., 1987. |
| Biological Activity | The lowest concentration which produces a clustered growth pattern with CHO cells is 0.03ng/ml. The adenylate cyclase activity of this preparation is |
| 20.1 picomoles/minute/ug in the presence of 1ug calmodulin. |
| Storage and Stability | Lyophilized powder may be stored at 4°C. Stable for 12 months at 4°C. Reconstitute with sterile ddH2O. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. |
| Source | Bordetella pertussis |
| Purity | ~99% (SDS-PAGE) |
| Concentration | ~0.1mg/ml (after reconstitution) |
| Form | Supplied as a lyophilized powder. from 10mM sodium phosphate, pH 7.0, 50mM sodium chloride. Reconstitute with 500ul sterile ddH2O. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
