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You are here:Home » Molecular Biology » MB-Infectious Disease » Bordetella pertussis Toxin

Bordetella pertussis Toxin

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Specifications

Islet-activating protein Pertussis toxin consists of an A protomer subunit (S1) which possesses both NAD+ glycohydrolase and ADP-ribosyltransferase activities, and B oligomer subunits (S2, S3, S4, and S5) which are responsible for attachment of the native toxin to eukaryotic cell surfaces. Pertussis toxin uncouples G proteins from receptors by ADP ribosylating a cysteine residue near the carboxyl terminus of the a subunit.
Catalog #B2558-55
This preparation migrates as five distinct bands when analyzed by SDS-Urea PAGE. The five bands correspond to one A protomer subunit, designated S1 (Mr=26.2kD), and four B oligomer subunits, designated S2-S5 (Mrs= 21.9, 21.9, 12.1, and 10.9kD, respectively).
ReconstitutionReconstitute with 500ul sterile ddH2O. Because Pertussis toxin is relatively insoluble, the resulting suspension should be made uniform by gentle mixing prior to withdrawing aliquots. Do not sterile filter.
This preparation is not activated. For use with intact cells or extracts, activation is not necessary. However, when used with purified G proteins, this preparation should be activated according to the procedure of Kaslow et al., 1987.
Biological ActivityThe lowest concentration which produces a clustered growth pattern with CHO cells is 0.03ng/ml. The adenylate cyclase activity of this preparation is
20.1 picomoles/minute/ug in the presence of 1ug calmodulin.
Storage and StabilityLyophilized powder may be stored at 4°C. Stable for 12 months at 4°C. Reconstitute with sterile ddH2O. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
SourceBordetella pertussis
Purity~99% (SDS-PAGE)
Concentration~0.1mg/ml (after reconstitution)
FormSupplied as a lyophilized powder. from 10mM sodium phosphate, pH 7.0, 50mM sodium chloride. Reconstitute with 500ul sterile ddH2O.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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