| Borrelia burgdorferi is a spirochete and the cause of Lyme disease, a tick transmitted illness of humans and animals. B. burgdorferi may persist in humans and animals for months or years following initial infection, despite a robust humoral immune response. |
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| Catalog # | B2570-12 |
| B. burgdorferi resembles other spirochetes in that it is a highly specialized, motile, two-membrane, spiral shaped bacteria which lives primarily as an extracellular pathogen. B. burgdorferi has an unusual genome compared with other eubacteria which includes a linear chromosome approximately one megabase in size and numerous linear and circular plasmids. |
| Applications | Suitable for use in ELISA, Fluorescence Microscopy, Western Blot, Immunohistochemistry and Immunocytochemistry. It is also suitable for Conjugation purposes. Other applications not tested. |
| Recommended Dilutions | Western Blot: >1:1,000 |
| Avidin-biotin IFA: >1:40,000 |
| Immunohistochemistry: Formalin-fixed paraffin-embedded sections |
| Optimal dilutions to be determined by the researcher. |
| Storage and Stability | May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Concentration | ~4-5mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. No stabilizing proteins have been added. |
| Purity | Purified IgG fraction to a purity of ~95%. |
| Immunogen | Whole cell preparation from B. burgdorferi. |
| Specificity | Recognizes Borrelia burgdorferi at 83kD, 41kD, 34kD and 31kD, and additional low MW bands. Crossreactivity: Treponema pallidum, B. hermsii & B. parkerii. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
