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You are here:Home » Molecular Biology » MB-Protein Kinases » BRSK2, N-terminal His-Tag, Recombinant, Human (BR Serine/threonine Kinase 2, SAD1, Homolog of SAD-1 (C. elegans), PEN11B, Serine/threonine Kinase 29, STK29, Chromosome 11 Open Reading Frame 7, C11orf7)

BRSK2, N-terminal His-Tag, Recombinant, Human (BR Serine/threonine Kinase 2, SAD1, Homolog
of SAD-1 (C. elegans), PEN11B, Serine/threonine Kinase 29, STK29, Chromosome 11 Open
Reading Frame 7, C11orf7)

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Specifications

Catalog #B2673-76R
SourceRecombinant corresponding to full length human BRSK2 with N-terminal His tag expressed in baculovirus expression system (NM_003957).
Specific Activity412pmoles/min/ug. Enzyme reaction is conducted in a buffer containing 50mM HEPES, pH 7.5, 10mM MgCl2, 1mM EGTA, 200uM ATP, 0.01% Brij-35 and 2uM substrate (SER/THR 21) at RT for 1 hour.
ApplicationsSuitable for use in the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested.
Recommended DilutionOptimal dilutions to be determined by the researcher.
Storage and StabilityAliquot to avoid repeated freezing and thawing. Store at -70°C. Aliquots are stable for at least 6 months at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight75kD
SourceBaculovirus
PurityPurified 70%
FormSupplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM sodium chloride, 0.05% tween-20, 10% glycerol.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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