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You are here:Home » Molecular Biology » Cloning-Primers » Brachyury (Brachyury Homolog, Brachyury Protein, Bry, MGC104817, T, T Protein, TFT), Mouse, Rat Primer Pair

Brachyury (Brachyury Homolog, Brachyury Protein, Bry, MGC104817, T, T Protein, TFT),
Mouse, Rat Primer Pair

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Specifications

Mouse/rat Brachyury specific PCR primers for RT-PCR (reverse transcription followed by polymerase chain reaction) analysis of mRNA expression.
Catalog #B2674-75C
The Brachyury protein is required for the proper formation and differentiation of posterior mesoderm and for axial development in all vertebrates. The Brachyury gene encodes a transcription factor that binds to a specific DNA element via its N-terminal region. A protein motif within the DNA-binding domain, the so-called T box, is highly conserved among T homologs from different species and also defines a broader family of T-box genes Brachyury functions during the earlier stages of gastrulation, which suggests that at least one of its early roles is to regulate the morphogenetic behavior of nascent mesoderm by altering cell autonomous properties.
ComponentsPrimer Pair: Lyophilized. Each vial contains 375 pmoles of each primer. Adjust to a final concentration of 7.5 pmoles/ul by resuspending Primer Pair in either 50ul autoclaved deionized water or 0.1X TE buffer (1mM Tris HCl, pH8.0 @ 25°C; 0.1mM EDTA, pH 8.0 @ 25°C).
Positive Control 60 Lyophilized. Each vial contains 150 ng synthetic double-stranded DNA. Resuspend in 30ul autoclaved deionized water or TE buffer. To prevent contaminating samples and reagents, it is strongly recommended that the Positive Control is resuspended in a separate location from where PCR reactions are set up. Use a different pipette than one used for PCR set up. The Positive Control is not intended for quantitative purposes.
GenBank Accession NumberNM_009309 (mouse) and XM_217890 (rat)
ComponentscDNA: 216 base pairs (bp) (mouse and rat)
Genomic DNA: 1769 bp product predicted and observed for mouse. Sequence not available for rat. Product observed at 1769 bp for rat.
Positive Control 60: 290 bp
Alternate splice variants: None reported
Suggested Reaction ConditionsFor each 50ul Polymerase Chain Reaction mixture, 2ul of the primer pair stock solution should be used. Sufficient reagent is supplied for 25 separate amplification reactions of the gene fragment.
Thermocycling ConditionsA typical 50ul PCR would involve a 4 minute denaturation step at 94ºC, followed by 30-35 cycles of 45 seconds at 94ºC, 45 seconds at 55ºC and 45 seconds at 72ºC, with a final 10 minute extension step at 72ºC.
Storage and StabilityThe Primer Pair resuspended in water or TE buffer is stable for up to one year at -20°C in a non-frost free freezer. Aliquot in single use portions. Do not use past the expiration date above. Avoid repeated freeze-thaw cycles.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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