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You are here:Home » Kits and Assays » Kits for Molecular Biology, BioAssay™ » B-Raf Kinase Assay Kit, Chemiluminescence, BioAssay™ (BRAF, Ras effector)

B-Raf Kinase Assay Kit, Chemiluminescence, BioAssay™ (BRAF, Ras effector)

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Specifications

B-Raf Kinase Assay Kit is designed to measure B-Raf dependent phosphotransferase activity in a kinase reaction using recombinant MEK1, unactive as a B-Raf substrate. The enzyme assay is rapid, convenient and specific for the Raf family of kinases.
Catalog #B2680-02A
50 kinase assays per kit.
B-Raf Kinase Cascade ReactionMEK1, unactive + ATP ----- Active B-Raf, 30°C, 30 min ----> Active MEK1 + ADP
Kit ComponentsB2680-02A1:
B-Raf (delta1-415), active, Recombinant 1x10ug
B2680-02A2:
MEK1 (human), unactive, Recombinant 1x50ug
B2680-02A3:
Assay Dilution Buffer I (ADBI) 1x1ml
B2680-02A4:
Magnesium/ATP Cocktail 1x1ml
B2680-02A5:
MEK1 (Ser218/222) / MEK2 (Ser222/226) phosphorylated 1x200ug
Storage and StabilityStore components at -70°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.


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