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B-Raf Kinase Assay Kit, Chemiluminescence, BioAssay™ (BRAF, Ras effector)
B-Raf Kinase Assay Kit, Chemiluminescence, BioAssay™ (BRAF, Ras effector)
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| B-Raf Kinase Assay Kit is designed to measure B-Raf dependent phosphotransferase activity in a kinase reaction using recombinant MEK1, unactive as a B-Raf substrate. The enzyme assay is rapid, convenient and specific for the Raf family of kinases. | | | Catalog # | B2680-02A | | 50 kinase assays per kit. | | B-Raf Kinase Cascade Reaction | MEK1, unactive + ATP ----- Active B-Raf, 30°C, 30 min ----> Active MEK1 + ADP | | Kit Components | B2680-02A1: | | B-Raf (delta1-415), active, Recombinant 1x10ug | | B2680-02A2: | | MEK1 (human), unactive, Recombinant 1x50ug | | B2680-02A3: | | Assay Dilution Buffer I (ADBI) 1x1ml | | B2680-02A4: | | Magnesium/ATP Cocktail 1x1ml | | B2680-02A5: | | MEK1 (Ser218/222) / MEK2 (Ser222/226) phosphorylated 1x200ug | | Storage and Stability | Store components at -70°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. | |
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