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You are here:Home » Molecular Biology » MB-Protein Kinases » BRAF, p50 (cdc37) Serine/Threonine Kinase, Recombinant, Human, N-terminal His-Tag

BRAF, p50 (cdc37) Serine/Threonine Kinase, Recombinant, Human, N-terminal His-Tag

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Specifications

Human BRAF (a.a. 432-725) with N-terminal His tag, MW 36kD, co-expressed with p50 in an Baculovirus infected Sf9 cell expression system.
Catalog #B2680-05A
ApplicationSuitable for use in the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications have not been tested.
Recommended Dilutions Optimal dilutions to be determined by the researcher.
Specific Activity As reported.
Kinase AssayActivity is tested using 1uM MEK1 (K97R) as substrate. Assay is performed in a 50ul reation in 10mM Tris, pH 7.4, 5mM magnesium chloride, 0.5mM EDTA, 0.5mM DTT, 5uM ATP, 5ug MEK1 (K97R) as substrate, and B2680-05A. 62ng of BRAF enzyme phosphorylated over 18% of 1uM MEK1 at RT in 1hr.
Storage and StabilityAliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.
Molecular Weight36kD
SourceRecombinant, human from Baculovirus infected Sf9 cells
Purity25% by SDS-PAGE
FormSupplied as a liquid in 25mM Tris-HCl, pH 8.0, 150mM sodium chloride, 0.05% Tween-20, 40% glycerol and 3mM DTT.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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