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Brain-Derived Neurotrophic Factor, Recombinant, Human (BDNF)
Brain-Derived Neurotrophic Factor, Recombinant, Human (BDNF)
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| BDNF is a member of the NGF family of neurotrophic growth factors. Like other members of this family, BDNF supports neuron proliferation and survival. BDNF can bind to a low affinity cell surface receptor called LNGFR, which also binds other neurotrophins such as NGF, NT-3 and NT-4. However, BDNF mediates its neurotrophic properties by signaling through a high affinity cell surface receptor called gp145/trkB. BDNF is expressed as the C-terminal portion of a 247 amino acid polypeptide precursor, which also contains a signal sequence of 18 amino acid residues and a propeptide of 110 amino acid residues. | | | Catalog # | B2700 | | Recombinant human BDNF is a 27.0kD homodimer of two 119 amino acid subunits linked by strong non-covalent interactions. Human and mouse BDNF sequences are identical. | | Biological Activity | Biological activity was determined by its ability to stimulate the proliferation of rat C6 cells. The expected ED50 for this effect is 0.5-1ug/ml. | | AA Sequence | MHSDPARRGE LSVCDSISEW VTAADKKTAV DMSGGTVTVL EKVPVSKGQL KQYFYETKCN PMGYTKEGCR GIDKRHWNSQ CRTTQSYVRA LTMDSKKRIG WRFIRIDTSC VCTLTIKRGR | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Country of Origin: | | USA | | Molecular Weight | 27.0kD | | Source | Human, recombinant protein expressed in E. coli | | Purity | 98% (SDS-PAGE, HPLC) Endotoxin: 0.1ng/ug (1EU/ug) | | Form | Supplied as a lyophilized powder. Reconstitute with sterile ddH2O. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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