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Brain-Derived Neurotrophic Factor, Recombinant, Human (BDNF)
Brain-Derived Neurotrophic Factor, Recombinant, Human (BDNF)
Pricing
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| Catalog # | B2700-01B | | Source | Recombinant correspnding to human Brain-Derived Neurotrophic Factor, expressed in E. coli. | | Molecular Weight | ~27kD | | Activity | ED50 is approximately equal to 50ng/ml. The biological activity was determined by measuring the dose dependent induction of choline acetyl transferase activity in rat basal forebrain primary septal cultures. A concentration of 50ng/ml is effective for most in vitro applications. The optimal concentration should be determined for each specific application. | | Endotoxin | 0.1ng/ug | | Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | ~27kD | | Source | E. coli | | Purity | ~98% (SDS-PAGE, HPLC) | | Concentration | Not determined | | Form | Lyophilized from 10mM sodium citrate, pH 4.0. Reconstitute with sterile dH2O to 0.1-1mg/ml. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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