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You are here:Home » Molecular Biology » Growth Factors-BDNF » Brain-Derived Neurotrophic Factor, Recombinant, Human (BDNF)

Brain-Derived Neurotrophic Factor, Recombinant, Human (BDNF)

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Specifications

Catalog #B2700-01B
SourceRecombinant correspnding to human Brain-Derived Neurotrophic Factor, expressed in E. coli.
Molecular Weight~27kD
ActivityED50 is approximately equal to 50ng/ml. The biological activity was determined by measuring the dose dependent induction of choline acetyl transferase activity in rat basal forebrain primary septal cultures. A concentration of 50ng/ml is effective for most in vitro applications. The optimal concentration should be determined for each specific application.
Endotoxin 0.1ng/ug
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~27kD
SourceE. coli
Purity~98% (SDS-PAGE, HPLC)
ConcentrationNot determined
FormLyophilized from 10mM sodium citrate, pH 4.0. Reconstitute with sterile dH2O to 0.1-1mg/ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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