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Brain-Derived Neurotrophic Factor, Recombinant, Human (BDNF)
Brain-Derived Neurotrophic Factor, Recombinant, Human (BDNF)
Pricing
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| Human Brain-Derived Neurotrophic Factor is a homodimer, non-glycosylated, polypeptide chain containing 2 x 119 amino acids. The sequence of the first five N-terminal amino acids was determined to be Met-His-Ser-Asp-Pro. Contains less than 1% dimers and aggregates. | | | Catalog # | B2700-01H | | Source | Recombinant Human Brain-Derived Neurotrophic Factor expressed in E. coli. | | Molecular Weight | 26,984 | | Activity | The ED50 as determined by the dose dependent induction of ACHE (acetylcholine esterase) in rat basal forebrain primary septal culture is 50ng/ml. | | Endotoxin | 0.1 ng/ug | | Storage and Stability | Lyophilized powder may be stored at -20°C. Reconstitute with ddH2O. Aliquot and store at -20°C. For long-term storage, add a carrier protein (0.1% HSA or BSA), Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Molecular Weight | ~26,984 | | Source | E. coli | | Purity | ~96% (HPLC, FPLC, reducing and non-reducing SDS-PAGE) | | Concentration | ~1mg/ml | | Form | Supplied as a lyophilized powder from 20mM sodium citrate buffer, pH 5.0. No preservative added. Reconstitute using sterile deionized water to a concentration 100ug/ml. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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