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You are here:Home » Molecular Biology » Growth Factors-BDNF » Brain-Derived Neurotrophic Factor, Recombinant, Human (BDNF, Abrineurin, MGC34632)

Brain-Derived Neurotrophic Factor, Recombinant, Human (BDNF, Abrineurin, MGC34632)

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Specifications

Catalog #B2700-01K
SourceRecombinant corresponding to 2x119aa of human Brain-derived Neurotrophic Factor expressed in E.coli is a homodimer, non-glycosylated, polypeptide chain.
Molecular Weight~27kD
Biological ActivityThe ED50, calculated by the dose-dependent induction of ACHE (acetylcholine esterase)in rat basal forebrain primary septal culture is 50nug/ml
Dimers and Aggregates 1% (SDS-PAGE)
Protein ContentProtein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280nm using the absorbency value of 1.6 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of BDNF as a Reference Standard.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O, HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight~27kD
Purity~96% (RP-HPLC, Anion-exchange FPLC, SDS-PAGE) Endotoxin: 0.1ng/ug (IEU/ug)
ConcentrationNot determined
FormSupplied as a lyophilized powder. No preservative added. Reconstitute with sterile dH2O, 0.1% HSA or BSA to 0.1mg/ml.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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