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You are here:Home » Antibodies » Abs to Growth Factors, Cytokines » Anti -Brain Derived Neurotrophic Factor (BDNF)

Anti -Brain Derived Neurotrophic Factor (BDNF)

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified E B
Catalog #B2700-02A
ApplicationsSuitable for use in ELISA and Western Blot. Other applications not tested.
Recommended DilutionELISA: To detect hBDNF by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2-0.4ng/well of recombinant hBDNF.
Western Blot: To detect hBDNF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBDNF is 1.5-3.0ng/lane, under either reducing or non-reducing conditions.
Optimal dilutions to be determined by the researcher.
ReconstitutionReconstitute to 0.2mg/ml with sterile dH2O or sterile 40-50% glycerol for long term storage.
Storage and StabilityLyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Country of OriginUSA
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a lyophilized powder.
PurityPurified by immunoaffinity chromatography.
ImmunogenHighly pure (> 98%) recombinant human Brain-Derived Neurotrophic Factor
SpecificityRecognizes human Brain-Derived Neurotrophic Factor.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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