| BDNF (brain-derived neurotrophic factor) is a potent neurotrophin and member of the NGF-beta family, secreted from dense core vesicles, which promotes the growth, differentiation and survival of neurons within, or directly connected to, the central nervous system (CNS). BDNF signals through binding to the high affinity receptor trkB, and also binds to the low affinity neurotrophin receptor LNGFR. Studies have shown the involvement of BDNF in the modulation of short-term synaptic function and in the formation of long-term memory, and reduced levels of BDNF are seen in patients with Alzheimer's and Huntington disease. |
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| Catalog # | B2700-02D |
| Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. |
| Recommended Dilutions | ELISA: 0.5-2ug/ml |
| Western Blot: 0.1-0.2ug/ml |
| Optimal dilutions to be determined by the researcher. |
| Recommended Pair | Can be used in indirect ELISA or as capture antibody in sandwich ELISA with B2700-02B2 as the detection antibody. |
| Recommended Secondary Antibodies | I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb |
| I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb |
| I1904-46Q: IgG, H&L (HRP) Pab Gt xRb |
| Storage and Stability | Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Source | Human |
| Concentration | ~1mg/ml (after reconstitution) |
| Form | Supplied as a lyophilized powder from PBS, pH 7.2. No preservatives added. Reconstitute with 100ul sterile ddH2O. |
| Purity | Purified by affinity chromatography. |
| Immunogen | Recombinant human BDNF. |
| Specificity | Recognizes human Brain Derived Neurotrophic Factor (BDNF). |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
