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You are here:Home » Antibodies » Abs to Growth Factors, Cytokines » Anti -Brain Derived Neurotrophic Factor (BDNF)

Anti -Brain Derived Neurotrophic Factor (BDNF)

Pricing

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Specifications

Clone Host Grade Applications
Monoclonal Mouse Affinity Purified E B
Brain-derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors that includes NGF, NT-3, and NT-4. All neurotrophins have six conserved cysteine residues and share a 55% sequence identity at the amino acid level. BDNF has been shown to enhance the survival and differentiation of several classes of neurons in vitro, including neural crest and placode-derived sensory neurons, dopaminergic neurons in the substantia nigra, basal forebrain cholinergic neurons, hippocampal neurons, and retinal ganglial cells.1 BDNF is expressed within peripheral ganglia and is not restricted to neuronal target fields, raising the possibility that BDNF has paracrine or even autocrine actions on neurons as well as non-neuronal cells.2
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.
Catalog #B2700-02L
Recommended DilutionsWestern Blot: 1:100-1:2000 detects BDNF at ~300ng/lane under non-reducing and reducing conditions.
Optimal dilutions to be determined by the researcher.
Storage and StabilityMay be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clone TypeMonoclonal
IsotypeIgG1
Clone No8J359
HostMouse
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a liquid in PBS, pH 7.2.
PurityPurified by Protein A affinity chromatography.
ImmunogenRecombinant protein corresponding to human BDNF expressed in Sf21 insect cells. Cellular Localization: Secreted
SpecificityRecognizes human BDNF. Will neutralize the biological activity of rhBDNF. Shows <2% crossreactivity with rhbeta-NGF, rhNT-3 and rhNT-4 based on ELISA and Western Blot.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesAbrineurin


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