| Brain Derived Neurotrophic Factor (BDNF) kit is a sandwich enzyme immunoassay (EIA), which measures BDNF. Kit is designed to measure the amount of BDNF in cell culture supernatants, tissue homogenates and biological fluid (serum, plasma, and serum-free) samples from human and rat. With the BDNF assay system, rabbit polyclonal antibodies generated against human BDNF are coated onto a microplate and are used to capture BDNF from a sample. BDNF specific, biotin conjugated, mouse monoclonal antibodies detect the captured BDNF. After addition of streptavidin, enzyme, substrate and stop solution the amount of BDNF is determined. The standard curve demonstrates a direct relationship between Optical Density (OD) and BDNF concentration, i.e., the higher the OD the higher the BDNF concentration in the sample. |
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| Catalog # | B2700-08 |
| Sensitivity | 7.8pg/ml |
| Range of Detection | 7.8pg/ml to 500pg/ml |
| Crossreactivity | No significant crossreactivity with NGF, NT4/5 or NT3 |
| Intra-assay Variation | ± 3.7% (125pg/ml) |
| Inter-assay Variation | ± 8.5% (125pg/ml) |
| Kit Components | There are enough reagents included in this kit for two 96-well immuno-assay plates. |
| B2700-08A: BDNF ELISA Plates, 2x1 plate Pre-coated with Rabbit anti-Human BDNF Polyclonal Antibody 96-Well Microtiter plates. |
| B2700-08B: Wash Buffer, 10X, 1x100ml |
| B2700-08C: Standard/Sample Diluent, 1x60ml |
| *B2700-08D: BDNF Standard (Recombinant Human), 2x1 vials (Lyophilized). |
| B2700-08E: Mab (Biotin), 1x25ul |
| B2700-08F: Streptavidin (HRP), 1x50ul |
| B2700-08G: TMB/E Solution, 2x10ml |
| B2700-08H: Stop Solution (HCl), 1x22ml |
| Storage and Stability | Store at 4°C. Do not freeze. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. |
| *B2700-08D: BDNF Standard, Store at 4°C for up to 30 days (liquid). |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
