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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified B

Brain natriuretic peptide is a 32 amino acid polypeptide secreted by the ventricles of the heart in response to excessive stretching of myocytes in the ventricles. At the time of release, a co-secreted 76 amino acid N-terminal fragment (NT-proBNP) is also released with BNP. BNP binds to and activates NPRA in a similar fashion to atrial natriuretic peptide (ANP) but with 10-fold lower affinity.
Catalog #	B2702
Applications	Suitable for use in Westen Blot Other applications have not been tested.
Recommended Dilution	Western Blot: 0.5ug/ml detects BNP in 10ug rat spleen lysate.
	Optimal dilutions to be determined by the researcher.
Recommended Secondary AntibodiesI1904-39	IgG, X-Adsorbed (HRP) Pab Gt xRb
	I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb
	I1904-46Q: IgG, H&L (HRP) Pab Gt xRb
Control	Rat Spleen Lysate.
Storage and Stability	May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
CAS Number	n/a
Clone Type	Polyclonal
Isotype	IgG
Host	Rabbit
Source	Rat
Concentration	~1mg/ml
Form	Supplied as a liquid in PBS, 0.1% sodium azide.
Purity	Purified by affinity chromatography.
Immunogen	Synthetic peptide corresponding to rat Brain Natriuretic Peptide.
Specificity	Recognizes rat Brain Natriuretic Peptide.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.