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You are here:Home » Molecular Biology » MB-Hormones, Steroids » Brain Natriuretic Peptide Pro, NT, Recombinant, Human (NT-proBNP, ProBNP, Brain Natriuretic Peptide 32, BNP 32, Gamma Brain Natriuretic Peptide, Natriuretic Peptide Brain Type, Natriuretic Peptide Precursor B, Natriuretic Peptides B, NPPB Protein)

Brain Natriuretic Peptide Pro, NT, Recombinant, Human (NT-proBNP, ProBNP, Brain
Natriuretic Peptide 32, BNP 32, Gamma Brain Natriuretic Peptide, Natriuretic Peptide Brain
Type, Natriuretic Peptide Precursor B, Natriuretic Peptides B, NPPB Protein)

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Specifications

In cardiac tissue brain natriuretic peptide (BNP) is synthesized as 134aa precursor (prepro-BNP), which is cleaved by proteases to form a 26aa "signal" peptide and a 108aa pro-BNP. Proteolytic digestion of pro-BNP results in formation of 76aa amino-terminal NT-proBNP and biologically active 32aa BNP hormone molecule. Both proBNP and NTproBNP circulate in human plasma and have been proposed as markers for early diagnosis of left ventricular dysfunction as well as prognostic markers of possible cardiac complications at patients with heart failure.
Catalog #B2702-01E
ApplicationsSuitable for use in ELISA. Other applications have not been tested.
Recommended DilutionsOptimal dilutions to be determined by the researcher.
SpecificityImmunoreactivity is confirmed by reaction with monoclonal antibodies specific to different fragments of NT-proBNP molecule. Predicted molecular mass 8.6kD confirmed by MALDI-MS. Contains an additional methionine residue at the N-terminus (in comparison with native NT-proBNP). Does not contain a fusion partner.
Storage and StabilityAliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight8.6kD
SourceE. coli
PurityPurifited by Ion-exchange chromatography. ~95% (Tricine-SDS-PAGE).
Concentration~0.9mg/ml
FormSupplied as a liquid in PBS, pH 7.4.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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