| Catalog # | B2702-04R |
| Hybridoma | Clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with Synthetic NT-proBNP peptide, corresponding to aa61-76. |
| Applications | NT-proBNP and proBNP immunoassay. Suitable for use in Western Blot and ELISA. Other applications not tested. |
| Recommended Dilution | Optimal dilutions to be determined by the researcher. |
| Recommended Pair | Coating – Conjugate: |
| B2702-04F – B2702-04P, B2702-04Q – B2702-04P, B2702-04Q – B2702-04R; The pairs recognize the antigen in blood of patients with unstable angina and myocardial infarction. |
| Mabs, specific to other epitopes, could also be used in sandwich immunoassay. Possible Mab combinations tested with antigen from patient's blood | B2702-04Q – B2702-04S, B2702-04D – B2702-04P, B2702-04K – B2702-04P, B2702-04T – B2702-04H, B2702-04T – B2702-04S |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG2a |
| Clone No | 5E863 |
| Host | Mouse |
| Source | Human |
| Concentration | As reported |
| Form | Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide. |
| Purity | Purified by Protein A affinity chromatography. |
| Immunogen | Synthetic NT-proBNP peptide, corresponding to aa61-76. The peptide used for immunization was conjugated with carrier protein. |
| Specificity | Human proBNP and NT-proBNP. |
|
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
| Alternate names | NT-proBNP, N-terminal prohormone brain natriuretic peptide |
