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You are here:Home » Antibodies » Abs to Peptides » Anti -Brain Natriuretic Peptide, Pro, NT (BNP)

Anti -Brain Natriuretic Peptide, Pro, NT (BNP)

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Specifications

Clone Host Grade Applications
Monoclonal Rat Affinity Purified E IH
Brain-type Natriuretic Peptide (BNP) is a nonglycosylated peptide that is produced predominantly by ventricular myocytes and belongs to the natriuretic peptide family. Proteolytic cleavage of the 12kD BNP precursor gives rise to N-terminal proBNP (NT-proBNP) and mature BNP. Plasma NT-proBNP is a marker for congestive heart failure, while mature BNP (aa103-134) promotes vasodilation and fluid and sodium excretion.
Catalog #B2702-04W
ApplicationsSuitable for use in ELISA and Immunohistochemistry. Other applications not tested.
Recommended DilutionDirect ELISA: 0.5-1ug/ml. The detection limit is ~20ng/well for recombinant human BNP.
Immunohistochemistry: 25ug/ml detects proBNP in paraffin-embedded normal human heart tissue sections.
Optimal dilutions to be determined by the researcher.
Storage and StabilityLyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypeMonoclonal
IsotypeIgG2b
Clone No8H160
HostRat
SourceHuman
Concentration~0.5mg/ml
FormSupplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
PurityPurified by Protein G affinity chromatography.
ImmunogenE. coli-derived recombinant human proBNP (aa27-134, Accession #P16860). Species Sequence Homology: porcine (51%), mouse (29%).
SpecificityRecognizes human proBNP. Does not react with BNP-32 (aa103-134).
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Alternate namesBNP Precursor


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