| Human brain natriuretic peptide (BNP) is a secreted protein which is a member of the natriuretic peptide family. BNP is a cardiac hormone, which is synthesized as a pro-hormone (proBNP), and is proteolytically cleaved to release a biologically active fragment (BNP), and an inactive fragment (NT-proBNP) into the circulation. |
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| Catalog # | B2702-29G |
| BNP is predominantly secreted from the cardiac ventricles in response to volume and pressure overload, and results in a number of biological activities including natriuresis, diuresis, vasorelaxation, and inhibition of the sympathetic nervous system. A high concentration of BNP in the bloodstream is indicative of heart failure. |
| Applications | Suitable for use in ELISA and Western Blot. Other applications not tested. |
| Recommended Dilution | Optimal dilutions to be determined by the researcher. |
| Recommended Pair | Suitable for use as the capture or solid phase antibody. |
| Matched to B2702-29F as the detection or conjugate (label) antibody. |
| Hybridoma | Derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice. |
| Storage and Stability | May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| CAS Number | n/a |
| Clone Type | Monoclonal |
| Isotype | IgG1 |
| Clone No | 5E514 |
| Host | Mouse |
| Source | Human |
| Concentration | ~2mg/ml |
| Form | Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide, before the addition of glycerol to 40%. |
| Purity | Purified by Protein A affinity chromatography. |
| Immunogen | Synthetic BNP (whole molecule), conjugated with carrier protein |
| Specificity | Recognizes human BNP and proBNP. Also recognizes synthetic peptide 26CKVLRRH32. |
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| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
