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Specifications

Clone Host Grade Applications Monoclonal Mouse Affinity Purified E

Human brain natriuretic peptide (BNP) is a secreted protein which is a member of the natriuretic peptide family. BNP is a cardiac hormone, which is synthesized as a pro-hormone (proBNP), and is proteolytically cleaved to release a biologically active fragment (BNP), and an inactive fragment (NT-proBNP) into the circulation.
Catalog #	B2702-29K
	BNP is predominantly secreted from the cardiac ventricles in response to volume and pressure overload, and results in a number of biological activities including natriuresis, diuresis, vasorelaxation, and inhibition of the sympathetic nervous system. A high concentration of BNP in the bloodstream is indicative of heart failure.
Applications	Suitable for use in ELISA. Other applications not tested.
Recommended Dilution	Optimal dilutions to be determined by the researcher.
Hybridoma	Derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with synthetic BNP (whole molecule), conjugated with carrier protein.
Storage and Stability	May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
CAS Number	n/a
Clone Type	Monoclonal
Isotype	IgG2a
Clone No	5E517
Host	Mouse
Source	Human
Concentration	~5mg/ml
Form	Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Purity	Purified by Protein A affinity chromatography.
Immunogen	Synthetic BNP (whole molecule), conjugated with carrier protein
Specificity	Human BNP and proBNP.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.