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Specifications

Clone Host Grade Applications Polyclonal Rabbit Affinity Purified E B

Catalog #	B2705-01
Applications	Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution	ELISA: To detect hBRAK by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2-0.4ng/well of recombinant hBRAK.
	Western Blot: To detect hBRAK by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBRAK is 1.5-3.0ng/lane, under either reducing or non-reducing conditions.
	Optimal dilutions to be determined by the researcher.
Reconstitution	Reconstitute to 0.2mg/ml with sterile dH2O or sterile 40-50% glycerol for long term storage.
Storage and Stability	Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Country of Origin	USA
CAS Number	n/a
Clone Type	Polyclonal
Host	Rabbit
Source	Human
Concentration	~1mg/ml
Form	Supplied as a lyophilized powder.
Purity	Purified by immunoaffinity chromatography.
Immunogen	Highly pure (> 98%) recombinant human BRAK
Specificity	Recognizes human BRAK.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.