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You are here:Home » Antibodies » Abs to Cancer Markers » Anti -BRCA1, aa768-793 (Breast Cancer Antigen, Early Onset Breast Ovarian Cancer Susceptibility Protein 1)

Anti -BRCA1, aa768-793 (Breast Cancer Antigen, Early Onset Breast Ovarian Cancer
Susceptibility Protein 1)

Pricing

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Specifications

Clone Host Grade Applications
Polyclonal Rabbit Affinity Purified IP IH FC IF
BRCA1 contains at least two nuclear localization signals and is proposed to be a tumor suppressor protein. It is a serine phosphoprotein that undergoes hyper-phosphorylation during late G1-and S-phases of the cell cycle and is transiently dephosphorylated early after M-phase.1 BRCA1 protein alters in a qualitative and quantitative manner during cell cycle progression.1 The amount of BRCA1 protein is highest during S-phase and remains elevated toward G2/M, before it declines in early G1-phase.1 Inherited loss of BRCA1 function confers an increased susceptibility for both breast and ovarian cancer.
Catalog #B2709
ApplicationsSuitable for use in Flow Cytometry, Immunofluorescence and Immunoprecipitation. Other applications have not been tested.
Recommended DilutionImmunoprecipitation (Use Protein A):10ug/mg protein lysate. Co-immunoprecipitates EGFR.
Optimal dilutions to be determined by the researcher.
Positive ControlHeLa, HBL-100 (“normal” breast)1, or MDA-MB-468 cells.1
Cellular LocalizationNuclear
Storage and StabilityMay be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Clone TypePolyclonal
HostRabbit
SourceHuman
Concentration~1mg/ml
FormSupplied as a liquid in PBS, pH 7.4, 15mM sodium azide, 0.2% BSA. Also supplied without BSA and Sodium Azide. Refer to B2709X.
PurityPurified by Protein A affinity chromatography.
ImmunogenSynthetic peptide, aa768-793, (SISLVPGTDYGTQESISLLEVSTLGK) from the human BRCA1, conjugated to KLH.
SpecificityRecognizes BRCA1 (aa 768-793) of human.
Important NoteThis product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


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