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Anti -BRCA1, aa768-793 (Breast Cancer Antigen, Early Onset Breast Ovarian Cancer Susceptibility Protein 1)
Anti -BRCA1, aa768-793 (Breast Cancer Antigen, Early Onset Breast Ovarian Cancer
Susceptibility Protein 1)
Pricing
For pricing information, USA customers sign in. Outside USA? Please contact your distributor for pricing. Specifications
| Clone |
Host |
Grade |
Applications |
| Polyclonal |
Rabbit |
Affinity Purified |
IP IH FC IF |
|
| BRCA1 contains at least two nuclear localization signals and is proposed to be a tumor suppressor protein. It is a serine phosphoprotein that undergoes hyper-phosphorylation during late G1-and S-phases of the cell cycle and is transiently dephosphorylated early after M-phase.1 BRCA1 protein alters in a qualitative and quantitative manner during cell cycle progression.1 The amount of BRCA1 protein is highest during S-phase and remains elevated toward G2/M, before it declines in early G1-phase.1 Inherited loss of BRCA1 function confers an increased susceptibility for both breast and ovarian cancer. | | | Catalog # | B2709 | | Applications | Suitable for use in Flow Cytometry, Immunofluorescence and Immunoprecipitation. Other applications have not been tested. | | Recommended Dilution | Immunoprecipitation (Use Protein A):10ug/mg protein lysate. Co-immunoprecipitates EGFR. | | Optimal dilutions to be determined by the researcher. | | Positive Control | HeLa, HBL-100 (“normal” breast)1, or MDA-MB-468 cells.1 | | Cellular Localization | Nuclear | | Storage and Stability | May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. | | Clone Type | Polyclonal | | Host | Rabbit | | Source | Human | | Concentration | ~1mg/ml | | Form | Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide, 0.2% BSA. Also supplied without BSA and Sodium Azide. Refer to B2709X. | | Purity | Purified by Protein A affinity chromatography. | | Immunogen | Synthetic peptide, aa768-793, (SISLVPGTDYGTQESISLLEVSTLGK) from the human BRCA1, conjugated to KLH. | | Specificity | Recognizes BRCA1 (aa 768-793) of human. | | | Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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